500 error on multisite install

My wesbite yofnel.com is viewable When my htaccess is like this

# END WordPress

RewriteEngine On
RewriteBase /
RewriteRule ^index\.php$ - [L]

# add a trailing slash to /wp-admin
RewriteRule ^wp-admin$ wp-admin/ [R=301,L]

RewriteCond %{REQUEST_FILENAME} -f [OR]
RewriteCond %{REQUEST_FILENAME} -d
RewriteRule ^ - [L]
RewriteRule ^(wp-(content|admin|includes).*) $1 [L]
RewriteRule ^(.*\.php)$ $1 [L]
RewriteRule . index.php [L]

But if I remove
# END WordPress

It doesn't work anymore.
Please help, I'm frustrated on setting up my multisite.

Subdomains also dont work even if I have created a wildcard in cpanel